matrigel-coated 5-layer cell culture flasks (Corning Life Sciences)
90
Structured Review
Corning Life Sciences
matrigel-coated 5-layer cell culture flasks
Matrigel Coated 5 Layer Cell Culture Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/matrigel-coated+5-layer+cell+culture+flasks/matrigel+coated+flasks/bio_rxiv__2023__12__27__573461-160-59-64
Average 90 stars, based on 1 article reviews
Matrigel Coated 5 Layer Cell Culture Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/matrigel-coated+5-layer+cell+culture+flasks/matrigel+coated+flasks/bio_rxiv__2023__12__27__573461-160-59-64
Average 90 stars, based on 1 article reviews
matrigel-coated 5-layer cell culture flasks - by Bioz Stars,
2026-10
90/100 stars
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Transduction:Article Title: Comparative landscape of genetic dependencies in human and chimpanzee stem cells Article Snippet: CRISPRi PSCs expressing dCas9-KRAB were dissociated with Accutase (Innovative Cell Technologies, cat. AT104-500), resuspended in StemFlex supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Sigma-Aldrich, cat. TR-1003-G), transduced with the lentiviral hCRISPRi-v2 sgRNA library at a target infection rate of 25–40%, and plated in Matrigel-coated Article Title: Mapping cis - and trans -regulatory target genes of human-specific deletions Article Snippet: Chimpanzee CRISPRi iPS cells (C3624K or Pt5-C, hDel-v1; C3624K, hDel-v2) were dissociated with Accutase (Innovative Cell Technologies, AT104-500), resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v1 or hDel-v2 lentiviral sgRNA library at a target infection rate of 25%, and plated at a density of 85,000 cells/cm in Matrigel-coated Infection:Article Title: Comparative landscape of genetic dependencies in human and chimpanzee stem cells Article Snippet: CRISPRi PSCs expressing dCas9-KRAB were dissociated with Accutase (Innovative Cell Technologies, cat. AT104-500), resuspended in StemFlex supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Sigma-Aldrich, cat. TR-1003-G), transduced with the lentiviral hCRISPRi-v2 sgRNA library at a target infection rate of 25–40%, and plated in Matrigel-coated Article Title: Mapping cis - and trans -regulatory target genes of human-specific deletions Article Snippet: Chimpanzee CRISPRi iPS cells (C3624K or Pt5-C, hDel-v1; C3624K, hDel-v2) were dissociated with Accutase (Innovative Cell Technologies, AT104-500), resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v1 or hDel-v2 lentiviral sgRNA library at a target infection rate of 25%, and plated at a density of 85,000 cells/cm in Matrigel-coated Cell Culture:Article Title: Comparative landscape of genetic dependencies in human and chimpanzee stem cells Article Snippet: CRISPRi PSCs expressing dCas9-KRAB were dissociated with Accutase (Innovative Cell Technologies, cat. AT104-500), resuspended in StemFlex supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Sigma-Aldrich, cat. TR-1003-G), transduced with the lentiviral hCRISPRi-v2 sgRNA library at a target infection rate of 25–40%, and plated in Matrigel-coated Article Title: Mapping cis - and trans -regulatory target genes of human-specific deletions Article Snippet: Chimpanzee CRISPRi iPS cells (C3624K or Pt5-C, hDel-v1; C3624K, hDel-v2) were dissociated with Accutase (Innovative Cell Technologies, AT104-500), resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v1 or hDel-v2 lentiviral sgRNA library at a target infection rate of 25%, and plated at a density of 85,000 cells/cm in Matrigel-coated Saline:Article Title: Comparative landscape of genetic dependencies in human and chimpanzee stem cells Article Snippet: CRISPRi PSCs expressing dCas9-KRAB were dissociated with Accutase (Innovative Cell Technologies, cat. AT104-500), resuspended in StemFlex supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Sigma-Aldrich, cat. TR-1003-G), transduced with the lentiviral hCRISPRi-v2 sgRNA library at a target infection rate of 25–40%, and plated in Matrigel-coated Article Title: Mapping cis - and trans -regulatory target genes of human-specific deletions Article Snippet: Chimpanzee CRISPRi iPS cells (C3624K or Pt5-C, hDel-v1; C3624K, hDel-v2) were dissociated with Accutase (Innovative Cell Technologies, AT104-500), resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v1 or hDel-v2 lentiviral sgRNA library at a target infection rate of 25%, and plated at a density of 85,000 cells/cm in Matrigel-coated Expressing:Article Title: Comparative landscape of genetic dependencies in human and chimpanzee stem cells Article Snippet: CRISPRi PSCs expressing dCas9-KRAB were dissociated with Accutase (Innovative Cell Technologies, cat. AT104-500), resuspended in StemFlex supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Sigma-Aldrich, cat. TR-1003-G), transduced with the lentiviral hCRISPRi-v2 sgRNA library at a target infection rate of 25–40%, and plated in Matrigel-coated Article Title: Mapping cis - and trans -regulatory target genes of human-specific deletions Article Snippet: Chimpanzee CRISPRi iPS cells (C3624K or Pt5-C, hDel-v1; C3624K, hDel-v2) were dissociated with Accutase (Innovative Cell Technologies, AT104-500), resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v1 or hDel-v2 lentiviral sgRNA library at a target infection rate of 25%, and plated at a density of 85,000 cells/cm in Matrigel-coated Transfection:Article Title: Comparative landscape of genetic dependencies in human and chimpanzee stem cells Article Snippet: CRISPRi PSCs expressing dCas9-KRAB were dissociated with Accutase (Innovative Cell Technologies, cat. AT104-500), resuspended in StemFlex supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Sigma-Aldrich, cat. TR-1003-G), transduced with the lentiviral hCRISPRi-v2 sgRNA library at a target infection rate of 25–40%, and plated in Matrigel-coated Article Title: Mapping cis - and trans -regulatory target genes of human-specific deletions Article Snippet: Chimpanzee CRISPRi iPS cells (C3624K or Pt5-C, hDel-v1; C3624K, hDel-v2) were dissociated with Accutase (Innovative Cell Technologies, AT104-500), resuspended in v3.1 medium supplemented with 2 μM thiazovivin and 5 μg/ml polybrene (Mirus, MIR 6620), transduced with the hDel-v1 or hDel-v2 lentiviral sgRNA library at a target infection rate of 25%, and plated at a density of 85,000 cells/cm in Matrigel-coated |